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Table 4 Inhibitory activities of the mushroom HAEs and ASEs during screening, attachment and penetration assays

From: Anti-viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: an in-vitro study

 

Screening

Attachment

Penetration

IC50 (μg/ml)

SI

IC50 (μg/ml)

SI

IC50 (μg/ml)

SI

P. giganteus HAE

344.8 ± 35.4**

12.4 ± 2.2**

–

–

1731 ± 160**

2.5 ± 0.3**

L. rhinocerotis HAE

485.9 ± 69.5**

7.4 ± 0.4**

–

–

–

–

S. commune HAE

312.9 ± 14.1**

13.7 ± 4.8*

–

–

943.1 ± 70.4**

4.5 ± 0.4**

H. erinaceus HAE

680.6 ± 79.3**

7.8 ± 2.6*

–

–

2080.2 ± 252.7**

2.5 ± 0.3**

P. giganteus ASE

637.9 ± 40.3**

15.7 ± 1**

872 ± 63.2**

11.5 ± 0.9**

315.4 ± 52.4**

32.3 ± 5.7**

L. rhinocerotis ASE

399.2 ± 18.9**

7.6 ± 0.6**

261.2 ± 38.3**

11.9 ± 1.7**

226.3 ± 157.1

17.5 ± 8.7

S. commune ASE

424.9 ± 76.6**

24.1 ± 4.4**

1245.8 ± 73.7**

8.1 ± 0.5**

279.3 ± 27.3**

36 ± 3.7**

H. erinaceus ASE

574.4 ± 83.4**

17.7 ± 2.7**

327.6 ± 29.2**

30.7 ± 2.9**

278.7 ± 87*

38.2 ± 11.4*

Ribavirin

80.7 ± 1.8**

10.9 ± 2.8**

–

–

205 ± 15.2**

4.3 ± 0.5**

  1. The anti-DENV2 activities of the mushroom extracts in Vero cells were evaluated in three different assays: simultaneous, attachment and penetration by the plaque reduction assay. The IC50 value, the concentration of extract required to inhibit 50% of virus growth compared with the virus control group, was calculated from the dose response curve of three independent experiments using probit analysis (n = 3). The selectivity index (SI) was calculated as the CC50/IC50. Statistical differences compared to the untreated virus control group are noted with asterisk (*P < 0.05) or (**p < 0.01)