Fig. 3

Morphological observation of B. frutescens leaves extracts treated MCF-7 cells using AO/PI dual staining at X400 magnifications. a Observation of morphological changes in MCF-7 cells. Viable cells are green stained cells with intact nucleus, condensed chromatin marked by intense green stained chromatin, membrane blebbing indicated by the outgrowth of plasma membrane and apoptotic cells are characterised by nuclear disintegration and leakage of plasma membrane. b MCF-7 cells were either treated with DMSO vehicle control (control) or B. frutescens extracts (hexane, L90, L70, L50 and water) for 24, 48 and 72 h at their respective IC₅₀ values