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Table 3 Metal chelating activity (MCA), ferric reducing-antioxidant power (FRAP), free radical scavenging capacities, total phenolic content, and total flavonoid content of different extracts of Jatu-Phala-Tiga

From: Traditional tonifying polyherbal infusion, Jatu-Phala-Tiga, exerts antioxidant activities and extends lifespan of Caenorhabditis elegans

Extracting solvents

MCA assay* (IC50; mg/mL)

FRAP assay (mM FeSO4/mg)

Radical scavenging properties** (IC50; mg/mL)

Total contents of (mg equivalence/g of extract)

DPPH

ABTS

Phenolics

Flavonoids

Ethyl acetate

48.62 ± 8.57b

14.22 ± 0.16b

0.99 ± 0.04c

0.59 ± 0.01c

350.20 ± 10.23b

78.25 ± 7.91a

Ethanol

216.09 ± 8.78c

23.07 ± 1.84a

0.41 ± 0.04b

0.27 ± 0.01a

457.81 ± 13.24a

68.06 ± 1.65ab

Water

1.75 ± 0.05a

16.81 ± 0.46b

0.31 ± 0.02a

0.31 ± 0.00b

253.01 ± 5.45c

64.92 ± 3.65b

  1. *IC50 of EDTA (a positive control) was 0.021 ± 0.00 mg/mL
  2. **IC50 of trolox obtained from DPPH and ABTS assays were 0.143 and 0.533 mg/mL, respectively
  3. a-cValues in the same column with different superscripts are significantly different (p < 0.05)