Fig. 2

Apoptosis induction effect of Huaier and 5-FU in single agent or combination on Huh28 cells. a Flow cytometry histograms of Huh28 cells after 48 h treatment with Huaier (6 mg/ml) and 5-FU (30 μg/ml) alone or in combination, the samples were detected using AV/PI double-staining method. b The percentage of apoptotic cells was calculated, and results are shown in bar graph. c, d and e The expression levels of anti-apoptotic protein Mcl-1 and Bcl-2 in Huh28 cells after 48 h of different treatments were detected by Western blot, and the relative optical densities of proteins were analyzed by Image J. All results are expressed as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared with control group, while ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared with combination group