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Table 1 Primer sequence for RT-PCR analysis

From: Water extract of Cnidii Rhizoma suppresses RANKL-induced osteoclastogenesis in RAW 264.7 cell by inhibiting NFATc1/c-Fos signaling and prevents ovariectomized bone loss in SD-rat

Target genes

Primer sequence

Accession number

Annealing

Cycle

Base pair

TRAP

F: 5′-act tcc cca gcc ctt act acc g-3′

R: 5′-tca gca cat agc cca cac cg-3′

NM_007388.3

58 °C

30

381

NFATc1

F: 5′-tgc tcc tcc tcc tgc tgc tc-3′

R: 5′-cgt ctt cca cct cca cgt cg-3′

NM_198429.2

58 °C

32

480

c-Fos

F: 5′-atg ggc tct cct gtc aac ac-3′

R: 5′-ggc tgc caa aat aaa ctc ca-3′

NM_010234.3

58 °C

33

480

RANK

F: 5′-aaa cct tgg acc aac tgc ac-3′

R: 5′-acc atc ttc tcc tcc cga gt-3′

NM_009399.3

53 °C

32

377

CA2

F: 5′-ctc tca gga caa tgc agt gct ga-3′

R: 5′-atc cag gtc aca cat tcc agc a-3′

NM_001357334.1

58 °C

32

411

CTK

F: 5′-agg cgg cta tat gac cac tg-3′

R: 5′-ccg agc caa gag agc ata tc-3′

NM_007802.4

58 °C

27

403

MMP-9

F: 5′-cga ctt ttg tgg tct tcc cc-3′

R: 5′-tga agg ttt gga atc gac cc-3′

NM_013599.4

58 °C

33

258

CTR

F: 5′-tgc att ccc ggg ata cac ag-3′

R: 5′-agg aac gca gac ttc act gg-3′

NM_001355192.1

59 °C

40

393

GAPDH

F: 5′-act ttg tca agc tca ttt cc-3′

R: 5′-tgc agc gaa ctt tat tga tg-3′

NM_008084.3

58 °C

30

267