Fig. 3

Effect of CR on osteoclast differentiation. The cells were stained with the TRAP assay kit, and media were collected for the TRAP activity. a TRAP-positive multinuclear cells were captured using an inverted microscope (100×, Scale bars: 200 μm). b Cells were counted, and c the media were measured for TRAP activity by an ELISA reader. Columns and error bars represent the mean ± S. E of three independent experiments. ^##p < 0.01 compared with normal and ^**p < 0.01, ^*p < 0.05 compared with the control