Fig. 6

The co exposure of fruit and seeds extracts of M. charantia resulted lethality and severe cardiac toxicity in zebrafish embryos. Representative photomicrograph showing live zebrafish embryos at 3dpf control or treated with fruit, seeds or combination of seeds and fruit extract of M. charantia. a The mock (methanol 0.5%V/V) exposed embryos did develop normally and had normal heart growth and development. b The zebrafish embryos treated with fruit extract (100 μg/ml) had severe cardiac hypertrophy, c the embryos treated with 15 μg/ml of seeds extract had cardiac hypertrophy and also enlarged cardiac cavity. d The embryos which were treated with combination of both extract showed severe effect on heart formation as fused ventricle and atrium represented as string and also enlarged heart cavity