Fig. 5

The fruit extract of M .Charantia effected the heart formation not the heart growth in treated zebrafish embryos. Representative live photomicrograph of zebrafish embryos at 72 hpf. a: Untreated control embryos at 72 hpf showing the normal formation and development of heart (red dots area represented by arrow). b: Zebrafish embryos which were treated with fruit extract of M. charantia at one cells stage exhibited the cardiac hypertrophy (red dots area and black arrow). c: The mock (methanol 0.5% v/v) treated embryos which were treated with solvent at one cell stage had normally heart development. d: Zebrafish embryos which were exposed to fruit extract of M. charantia at 48 hpf also had normal heart formation and development (red dot area). Abbreviation hpf: hours post fertilization. Scale bar 50 μm