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Table 3 Analytical parameters of LC-MS/MS method for the analysis of 15 phytochemicals

From: Quercus infectoria gall extracts reduce quorum sensing-controlled virulence factors production and biofilm formation in Pseudomonas aeruginosa recovered from burn wounds

No.

Analytes

RTa

Precursor ion (m/z)b

R2c

RSD %d

Recovery (%)

Ue

Intraday

Interdays

Intraday

Interdays

1

Protocatechuic acid

7.00

153.4

0.9909

0.0060

0.0060

1.0096

0.9988

0.0215

2

Chlorogenic acid

8.03

353.3

0.9910

0.0074

0.0055

0.9941

0.9999

0.0299

3

Luteolin-7-glucoside

13.20

447.0

0.9939

0.0052

0.0037

1.0014

1.0072

0.0086

4

Rutin

13.67

609.1

0.9902

0.0063

0.0070

1.0049

1.0037

0.0136

5

Hesperidin

13.68

611.1

0.9942

0.0081

0.0073

1.0053

0.9994

0.0162

6

Hyperoside

13.69

463.0

0.9905

0.0074

0.0056

1.0039

1.0015

0.0126

7

Apigetrin

14.54

431.0

0.9902

0.0047

0.0067

1.0060

1.0047

0.0132

8

Quercitrin

14.98

447.0

0.9918

0.0079

0.0063

0.9999

1.0002

0.0133

9

Astragalin

15.13

447.0

0.9900

0.0086

0.0077

1.0002

1.0017

0.0153

10

Quercetin

17.10

301.2

0.9962

0.0177

0.0227

1.0010

1.0012

0.0573

11

Luteolin

17.78

285.2

0.9901

0.0119

0.0079

0.9961

1.0007

0.0188

12

Apigenin

19.20

269.2

0.9910

0.0087

0.0090

0.9985

1.0022

0.0181

13

Pseudohypericin

26.34

519.0

0.9908

0.0061

0.0089

1.0033

1.0034

0.0172

14

Hyperforin

28.97

535.3

0.9901

0.0218

0.0164

1.0076

1.0061

0.0418

15

Hypericin

30.18

503.0

0.9925

0.0093

0.0095

1.0104

1.0034

0.0189

  1. aRT Retention time
  2. bPrecursor ion(m/z) Molecular ions of standard compounds
  3. cR2 Correlation coefficient
  4. dRSD Relative standard deviation
  5. eU (%) Percent relative uncertainty at 95% confidence level (k = 2)