Table 1 Primers of quantitation Real-time PCR (qRT-PCR) used in this study
Gene Name | Primer | Sequences (5′–3′) |
|---|---|---|
icaA | F | TGCTGGCGCAGTCAATACTA |
| Â | R | CATGGCAAGCGGTTCATACT |
icaR | F | CCTTATTTTCAGAGAAGGGGTATG |
| Â | R | CGAATACACTTCATCTTTGAATTG |
fnbA | F | GTCAAGTTATGGCGACAGGA |
| Â | R | GCGTCACTGTTGTAGGATCA |
lrgA | F | GTCGTGAAACAACAAAAAGACGCA |
| Â | R | TTAATCATGAGCTTGTGCCTCCTC |
lrgB | F | GCATCGTATCATCGGAGGTA |
| Â | R | GGTAACGCAATCGCTGTAGT |
cidA | F | ATATTGGCACAGAAATTCAAAAGA |
| Â | R | TCATAAGCGTCTACACCTTTACGA |
srrB | F | CGCTTGCCATTGTCCTTGAT |
| Â | R | CTTGGTCCATGCGATCCATA |
spa | F | AAGAAGACGGCAACAAGCCT |
| Â | R | AGGCTTGTTGCCGTCTTCTT |
nuc | F | ATGGACGTGGCTTAGCGTAT |
| Â | R | TAGCCAAGCCTTGACGAACT |
pyk a | F | AATGGTTGCACGTGGTGACA |
| Â | R | TAGATTGCGTTGGCAACGTC |