Fig. 2

PNS activated the PI3K/AKT/mTOR signaling pathway during LR in vivo. C57BL/6 J mice were administered PNS 7 days before PH. (a) The regenerated liver in mice treated with or without PNS 7 days after PH. (b) Quantification of the relative ratio of liver weight/body weight in mice treated with or without PNS at 2 d and 7 d after PH. Serum concentrations of ALT (c) and AST (d) at 2 d and 7 d after PH. (e) Quantification of PCNA-positive cells in mice treated with or without PNS at 2 d and 7 d after PH. (f) Representative liver sections stained with PCNA in mice treated with or without PNS at 2 d and 7 d after PH (original magnification: × 200, scale bars represent 100 μm). (g) Total and phosphorylated protein levels of PI3k, AKT and mTOR in mice treated with or without PNS at 2 d after PH. (H)(I)(J) Bar graph shows the quantification of the total and phosphorylated protein levels of PI3k, AKT and mTOR. Values represent the mean ± SD of at least three independent experiments. * P<0.05