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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Salidroside protects against ox-LDL-induced endothelial injury by enhancing autophagy mediated by SIRT1-FoxO1 pathway

Fig. 4

SIRT1 and FOXO1 mediate the protective effects of SAL on ox-LDL-induced endothelial injury, oxidative stress and autophagy. a Autophagy of HUVECs stained with an LC3 antibody under fluorescence microscopy. In cells with ox-LDL and SAL (100 μM), pretreatment with NAM (200 nM) or AS1842856 (100 nM) obviously reduced fluorescence intensity. b Quantification analysis shows that NAM and AS1842856 both significantly reduced the percentages of cells with LC3 dots. Autophagy inhibitor 3-Methyladenine (3-MA; 100 μM), NAM and AS1842856 all obviously abolish the protective effect of SAL on ox-LDL-induced endothelial injury (c), increase in MAD level (d), and decrease in SOD activity (e). Data are shown as mean ± SD (n = 6). ***P < 0.001 vs. control group; ###P < 0.001 vs. ox-LDL group; $$P < 0.01, $$$P < 0.001 vs. SAL (100 μM) group

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