Fig. 2

Effect of salidroside on autophagy and oxidative stress in ox-LDL-induced HUVECs. a Fluorescence microscopy after immunofluorescence staining with an LC3 antibody, followed by FITC-labeled secondary antibody (green, original magnification × 100). The nucleus was stained with DAPI (blue). b Quantification of fluorescence intensity of LC3, as presented by cells with LC3 dots. SAL decreases the NADPH oxidase activity (c), increases MDA level (d) and decreases SOD activity (e) in HUVECs exposed to ox-LDL (100 μg/mL). Data are shown as mean ± SD (n = 6). ***P < 0.001 vs. control group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. ox-LDL group