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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Shen Shuai IIRecipe attenuates renal injury and fibrosis in chronic kidney disease by regulating NLRP3 inflammasome and Sirt1/Smad3 deacetylation pathway

Fig. 3

SSR reduced Smad3 acetylation in 5/6(A/I) rats by regulating Sirt1/Smad3 deacetylation pathway. (a) The protein levels of Sirt1,TGF-β1 and TGF-βR1 were determined by immunoblotting. (b) The ratio of Sirt1,TGF-β1 and TGF-βR1 to GAPDH protein was calculated. (n = 6) (c) Representative images of IHC staining for Sirt1. 400 × magnification. (d) Quantitative analysis of Sirt1 positive area(n = 4)(e) Immunoprecipitation and western blot analysis of Sirt1 and Smad3 acetylation in each group. (f) The ratio of Sirt1 and acetylated Smad3 to precipitated Smad3 protein was calculated. (n = 4) Values are mean ± SD. *P < 0.05, **P < 0.01

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