Fig. 2

The effects of LA on nitric oxide synthesis in HUVECs. a HUVECs were treated with various concentrations of LA (0.5, 1, 5, 10, and 15 μM) for 24 h, followed by the Griess assay. Con - non-treated negative control (b) The expression of eNOS mRNA levels in HUVECs, the cells were treated with various concentrations of LA (0.5, 1, 5, 10, and 15 μM) for 24 h, followed by the measurement of eNOS mRNA expression levels using qRT-PCR. These data are normalized by the mRNA expression levels of GAPDH. c Measurement of the activity of LA against the L-N(g)-nitro-L-arginine methyl ester (L-NAME)-induced decrease of nitric oxide (NO) production. The cells were treated with 15 μM LA for 24 h, after which they were treated with 25 μM L-NAME for 24 h, followed by the Griess assay. Different letters indicate a statistically significant difference between each group at *P < 0.05, as determined by Duncan’s multiple-range test