Fig. 4

a Cells were treated with variable concentrations of TJFs (0.1 and 1%) for 24 h, pre-stained with Hoechst 33342 before fixation and permeabilization of cells and reacted with active-caspase 3(apoptosis inducer) primary antibody. Florescence detected by confocal microscope. b Pro-caspase-3 and cleaved caspase-3 Protein levels were determined by Western blot analysis. The β-actin probe served as protein-loading control