Fig. 6
From: Physalin A regulates the Nrf2 pathway through ERK and p38 for induction of detoxifying enzymes
Physalin A induces Nrf2 and target gene expression through ERK and p38 kinase activation. a Western blot analysis of Nrf2 expression in HepG2 cells pretreated with U0126 (MEK inhibitor), SB202190 (p38 inhibitor), LY294002 (PI3K inhibitor), and SP600125 (JNK inhibitor) for 1 h, followed by treatment with 20 μM physalin A for 4 h. b Western blot analysis of phospho-ERK and phospho-p38 expression in HepG2 cells treated with 20 μM physalin A for 0, 15, 30, 45, 60, 90, and 120 min. c Western blot analysis of Nrf2 target gene expression in HepG2 cells pretreated with U0126 and SB202190 for 1 h and then with 20 μM physalin A for 8 h