Fig. 5
From: Physalin A regulates the Nrf2 pathway through ERK and p38 for induction of detoxifying enzymes
Physalin A induces Nrf2 nuclear expression and activation. a Western blot analysis of Nrf2 expression in HepG2 cells. The cells were treated with 20 μM physalin A for 0, 2, 4, 8, 12, 24 h. b Western blot analysis of Nrf2 expression in the nuclear fraction. The cells were treated with 10 and 20 μM physalin A for 4 h. c Immunocytochemistry showing Nrf2 nuclear accumulation in HepG2 cells. The cells were treated with 20 μM physalin A for 4 h