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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Effect of Celastrus orbiculatus in inhibiting Helicobacter pylori induced inflammatory response by regulating epithelial mesenchymal transition and targeting miR-21/PDCD4 signaling pathway in gastric epithelial cells

Fig. 5

COE inhibits H. pylori-induced miR-21 elevation and PDCD4 reduction. (a) GES-1 cells were treated by COE for 24 h after stimulating by H. pylori for 2 h. The miR-21 expression was quantitated by realtime-PCR. (b) COE ameliorates the H. pylori -induced inhibition of PDCD4 3′-UTR reporter activity. GES-1 cells were transfected with renilla reporter construct (pGL3-PDCD4_3’-UTR), miR-21 inhibitor (100 nM), negative control (100 nM), and pGL3-promoters and treated with H. pylori for 2 h in the presence of COE (20 μg/mL). Cellular lysates were subjected to a luciferase reporter assay as described in Materials and Methods. The results are expressed as relative activity (relative luminescence units (RLU)) normalized to the luciferase activity in the vector control cells without treatment. Data presented in the bar graphs are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to H. pylori infection-induced group. ##P < 0.01, compared to negative control group

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