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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Effect of Celastrus orbiculatus in inhibiting Helicobacter pylori induced inflammatory response by regulating epithelial mesenchymal transition and targeting miR-21/PDCD4 signaling pathway in gastric epithelial cells

Fig. 4

Effect of COE on H. pylori infection-induced methylation status and expression of PDCD4 gene. (a) The methylation status of the PDCD4 promoter induced by COE determined via the MSP assay. U-non-methylated product; M-methylated product. (b) PDCD4 protein levels after COE treatment was detected by western blot analysis. The relative density was normalized to GAPDH, which was determined by densitometric analysis. (c) PDCD4 mRNA levels after COE treatment was detected by RT-PCR. The data was analyzed using the 2-∆∆Ct method. Each experiment from three independent experiments and values are expressed as means ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to H. pylori infection-induced group

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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