Fig. 2From: Effect of Celastrus orbiculatus in inhibiting Helicobacter pylori induced inflammatory response by regulating epithelial mesenchymal transition and targeting miR-21/PDCD4 signaling pathway in gastric epithelial cellsEffect of COE on H. pylori infection-induced EMT-related gene expression. (a) Protein expression levels of E-cadherin, N-cadherin and vimentin were evaluated by western blot. GAPDH was served as an internal control of protein level. The relative density of EMT markers was normalized to GAPDH, which was determined by densitometric analysis. (b) Relative mRNA levels of EMT-related gene were examined by RT-PCR. The data was analyzed using the 2-∆∆Ct method. Each experiment from three independent experiments and values are expressed as means ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to H. pylori infection-induced groupBack to article page