Fig. 4

Inhibition of PO-induced hyperuricemia by K-25. LLC-PK1 cells were stimulated with PO (0.25 mM) with or without pretreatment with K-25 (0.5 mg/mL). a After 24 h of incubation, OAT1, OAT3, URAT1, GLUT9, and XO were measured using an immunoblot assay. b LLC-PK1 cells were incubated with K-25 for 24 h, and the intracellular levels of OAT3 and GLUT9 transporters were analyzed using immunofluorescence analysis. Scale bar = 50 μm. c Effect of K-25 on XO inhibition activity. Values are represented as means ± SEM, *p < 0.05, versus the allopurinol group. The statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001) was determined using ANOVA with Bonferroni correction