Fig. 5

Effects of Tac or Rasv on the expression of mTOR and AMPK proteins in the Jurkat cell line. (a) Immunoblotting of p-Ampk, Ampk, p-mTOR,, and mTOR in the Jurkat cell line pretreated with Tac (1 ng/mL) or Resv (25, 50 nM) and then cultured under Th17-differentiation conditions. Stimulation of Jurkat cells under Th17-differentiation conditions activated phosphorylation of (b) AMPK, and (c) mTOR as detected by Western blotting and shown by the ratio of phosphorylated proteins to total proteins. Tac did not change p-AMPK or p-mTOR. In contrast, treatment with Resv resulted in the reduction of p-mTOR and an increase in p-AMPK. *P < 0.05 vs. Nil and ^†P < 0.05 vs. Th17 polarizing condition and ^‡P < 0.05 vs. Tac 1 ng/mL. Resv, Resveratrol; Tac, tacrolimus