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Fig. 9 | BMC Complementary and Alternative Medicine

Fig. 9

From: Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells

Fig. 9

Protective effect of SA, Rb1 and SA-Rb1 4:6 on EA.hy926 cells against H2O2-induced cell injury by inhibiting excessive intracellular ROS production. a Inhibitory effect of SA, Rb1 and SA-Rb1 4:6 on ROS production induced by H2O2 in EA.hy926 cells. The ROS expression level was estimated by cellular ROS detection assay kit manufacturer (Abcam, Australia). TBPH (from kit) was used as positive control in the assay. Results were shown as absorbance by a microplate reader, with the means ± SEM, n > 3. b Concentration-effect curves of ROS inhibitory effects of SA, Rb1 and SA-Rb1 4:6 generated by CompuSyn software. c Log (CI) values were plotted as a function of suppression fractional of ROS (Fa) by ‘CompuSyn’ software. Fa values correspond to % ROS inhibitory effect relative to control. Dotted line is the reference line, where Log (CI) value equals 0; solid line represents CI values at different Fa

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