Fig. 7
Protective effects of DS, SQ and DS-SQ 6:4 against H2O2-induced endothelial cell injury by attenuating excessively expressed ROS production. a Inhibitory effect of DS, SQ and DS-SQ 6:4 on ROS production induced by H2O2 in EA.hy926 cells. The ROS expression level was estimated by cellular ROS detection assay kit manufacturer (Abcam, Australia). TBPH (from kit) was used as negative control and gallic acid (10 μg/mL) was used as a positive control in the assay. Results were shown as absorbance by a microplate reader, with the means ± SEM, n > 3. *** P < 0.001 compared with H2O2 group. b Log (CI) values were plotted as a function of suppression fractional of ROS level (Fa) by ‘CompuSyn’ software. Dotted line is the reference line, where Log (CI) value equals to 0; solid line represents Log (CI) values at different Fa. Fa values correspond to percentage ROS inhibitory effect relative to control