Fig. 5

Effects of SA, Rb1 and SA-Rb1 4:6 in inhibiting LDH leakage on EA.hy 926 cells against cellular damage induced by Hcy-Ado-TNF. a Inhibitory activity (% relative to control) of SA, Rb1 and SA-Rb1 4:6 (50–150 μM) in inhibiting increased LDH activity (relative to control). Data shown are means ± SEM, n > 3. * P < 0.05 vs. stim group; *** P < 0.001 vs. stim group; ‘stim’ represents Hcy-Ado-TNF stimulation. b Concentration-effect curves of LDH inhibitory effects of SA, Rb1 and SA-Rb1 4:6 generated by CompuSyn software. c CI values were plotted as a function of suppression fractional of LDH leakage (Fa) by ‘CompuSyn’ software. Fa values correspond to % LDH inhibitory effect relative to control. Dotted line is the reference line, where CI value equals 1; solid line represents CI values at different Fa