Skip to main content

Table 5 Accuracy and precision of quantitative method for determination of compound 1 and compound 2 in ethanolic extract of root of B.montanum

From: Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

Compounds

Spiked concentration (μg/mL)

Concentration found (μg/mL; Mean ± SD)

RSD

%Accuracy

Intra-run (n = 3)

 Compound 1

15

14.94 ± 0.32

2.1

98.3–102.0%

25

24.97 ± 0.38

1.5

40

40.65 ± 0.39

1.0

 Compound 2

30

29.63 ± 0.11

0.4

98.3–102.3%

50

50.04 ± 1.18

2.4

80

81.24 ± 1.63

2.0

Inter-run (n = 9)

 Compound 1

15

14.91 ± 0.20

1.3

97.7–102.0%

25

24.87 ± 0.29

1.2

40

40.43 ± 0.44

1.1

 Compound 2

30

29.83 ± 0.40

1.3

98.0–103.0%

50

50.19 ± 0.87

1.7

80

81.38 ± 1.03

1.3