Fig. 5
From: Wu-Mei-wan protects pancreatic β cells by inhibiting NLRP3 Inflammasome activation in diabetic mice
Effect of WMW on the expression of IL-1β and other proinflammatory cytokines in the mouse pancreas. Homogenates of mouse pancreas were tested by ELISA for (a) IL-1 β; (b) IL-18; (c) MCP-1 α; and (d) TNF-α. Western blot analysis for IL-1β was performed (e). Band densities were converted to a bar graph. Immunofluorescence staining for insulin (red) and IL-1β (green) in pancreatic islets from wild-type, db/db, and db/db mice treated with HWMW are illustrated in (f). Nuclei were counterstained with DAPI (blue). Signals for IL-1β were calculated in bar graph (g). Scale: 25 μm. P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) (n = 6)