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Table 6 In vitro chromosome aberration test in chinese hamster lung cells with OE extract from cactus stem

From: An oral toxicity test in rats and a genotoxicity study of extracts from the stems of Opuntia ficus-indica var. saboten

Treatment Schedulea

Dose (μg/mL)

Ratio of Aberrant Metaphaseb

Cell Countsc

Mean

RICC(%)d

6–18 (+S)

0

 

0

4792

4689

4741

100

500

 

0

5024

4931

4978

108

1500

P

0

4996

4774

4885

105

3000

P

2

4405

4247

4326

86

3500

P

2

4158

4107

4133

80

4000

P

1

3528

3491

3510

59

4500

TTP

1

3054

2971

3013

42

5000

TTP

2

2796

2756

2776

34

B[a]P 20

 

30

3066

3116

3091

45

6–18 (-S)

0

 

0

5899

5757

5828

100

500

 

0

5525

5389

5457

91

1500

P

1

5001

4962

4982

79

1800

P

2

4243

4539

4391

65

2000

P

0

3508

3445

3477

42

3000

P

2

2942

2734

2838

26

4NQO 0.4

 

18

3226

3148

3187

35

24–0 (-S)

0

 

0

5600

5444

5522

100

500

 

1

5400

5479

5440

98

1500

P

1

4971

4971

4971

85

3000

P

1

4153

4072

4113

63

4000

P

0

3863

3842

3853

56

5000

TTP

0

3652

3670

3661

51

4NQO 0.4

 

21

2853

2994

2924

31

Initial cell count

Cell countsc

Mean

1754

1673

1872

1750

1762

  1. aTreatment time – Recovery time in the presence (+S) and absence (-S) of metabolic activation system
  2. bRatio of metaphase with chromosome aberrations. One culture/ dose was used
  3. Gaps excludes, 100 metaphases/ culture were examined
  4. cAfter harvesting mitotic cells, each culture was trypsinized and suspended with 0.5 mL of 0.1% trypsin and 5 mL of culture medium
  5. The cell suspensions of 0.4 mL per culture were diluted 50 times with 19.6 mL of Isoton sol. The cells in 0.5 mL Isoton sol. were counted twice/ culture using Coulter Counter model Z2
  6. Actual number of cells per flask = Mean Count × 550
  7. dRelative Increase in cell Count = ((Cell count of treated flask – Initial cell count) / (Cell count of the negative control flask – initial cell count)) × 100 (%)
  8. T: Turbidity at the end of the treatment
  9. P: Precipitation at the end of the treatment
  10. B[a]: benzo[a] pyrene, 4NQO: 4-Nitroquinoline-1-oxide