An oral toxicity test in rats and a genotoxicity study of extracts from the stems of Opuntia ficus-indica var. saboten

Han, Eun Hye; Lim, Mi Kyung; Lee, Sang Ho; Rahman, Md. Mahbubur; Lim, Young-Hee

doi:10.1186/s12906-019-2442-7

BMC Complementary Medicine and Therapies

Table 5 Bacterial reverse mutation assay

From: An oral toxicity test in rats and a genotoxicity study of extracts from the stems of Opuntia ficus-indica var. saboten

Test Strain	Chemical Treated	Dose (μg/plate)	Colonies/plate [factor]^a
Test Strain	Chemical Treated	Dose (μg/plate)	With S9 mix	Without S9 mix
TA100	Test article	0	114 ± 13	109 ± 3
		5	130 ± 7 [1.1]	115 ± 11 [1.1]
		15	98 ± 9 [0.9]	104 ± 0 [1.0]
		50	117 ± 13 [1.0]	113 ± 14 [1.0]
		150	126 ± 9 [1.1]	115 ± 10 [1.1]
		500	116 ± 12 [1.0]	116 ± 3 [1.1]
		1500	121 ± 0 [1.1]	121 ± 7 [1.1]
		3000	133 ± 4 [1.2]	120 ± 11[1.1]
		5000	134 ± 3 [1.2]	104 ± 6 [1.0]
TA1535	Test article	0	12 ± 1	12 ± 2
		5	11 ± 1 [0.9]	11 ± 1 [0.9]
		15	10 ± 1 [0.8]	12 ± 4 [1.0]
		50	12 ± 1 [1.0]	13 ± 3 [1.1]
		150	9 ± 1 [0.7]	10 ± 1 [0.8]
		500	12 ± 3 [1.0]	14 ± 4 [1.2]
		1500	11 ± 1 [0.9]	14 ± 4 [1.2]
		3000	9 ± 0 [0.8]	10 ± 1 [0.9]
		5000	12 ± 3 [1.0]	11 ± 2 [0.9]
TA98	Test article	0	26 ± 4	24 ± 4
		5	27 ± 4 [1.0]	20 ± 1 [0.8]
		15	23 ± 2 [0.9]	21 ± 1 [0.9]
		50	25 ± 2 [1.0]	26 ± 6 [1.1]
		150	27 ± 4 [1.0]	25 ± 1 [1.0]
		500	30 ± 2 [1.2]	24 ± 3 [1.0]
		1500	27 ± 1 [1.1]	23 ± 1 [1.0]
		3000	29 ± 1 [1.1]	23 ± 3 [1.0]
		5000	31 ± 4 [1.2]	27 ± 4 [1.1]
TA1537	Test article	0	11 ± 1	9 ± 1
		5	10 ± 2 [0.9]	8 ± 0 [0.9]
		15	10 ± 1 [0.9]	8 ± 3 [0.9]
		50	10 ± 2 [0.9]	9 ± 4 [1.0]
		150	11 ± 4 [1.0]	7 ± 2 [0.8]
		500	14 ± 4 [1.3]	8 ± 2 [0.9]
		1500	12 ± 2 [1.1]	7 ± 1 [0.8]
		3000	9 ± 0 [0.9]	7 ± 1 [0.8]
		5000	9 ± 0 [0.9]	7 ± 1 [0.8]
E. coli WP2 uvrA	Test article	0	29 ± 0	22 ± 4
		5	23 ± 1 [0.8]	27 ± 1 [1.2]
		15	24 ± 4 [0.8]	23 ± 4 [1.1]
		50	29 ± 4 [1.0]	23 ± 3 [1.1]
		150	26 ± 1 [0.9]	21 ± 3 [1.0]
		500	25 ± 1 [0.9]	26 ± 2 [1.2]
		1500	25 ± 6 [0.9]	24 ± 4 [1.1]
		3000	26 ± 0 [0.9]	21 ± 4 [1.0]
		5000	23 ± 1 [0.8]	26 ± 8 [1.2]
Positive controls		(μg/plate)
TA100	2-AA	1.0	1588 ± 14 [13.7]
TA1535	2-AA	2.0	183 ± 2 [15.2]
TA98	B[a]P	1.0	159 ± 6 [6.2]
TA1537	2-AA	1.0	239 ± 1 [22.8]
WP2 uvrA	2-AA	6.0	143 ± 11 [4.9]
TA100	SA	0.5		408 ± 5 [3.7]
TA1535	SA	0.5		286 ± 6 [24.8]
TA98	2-NF	2.0		208 ± 11[8.7]
TA1537	ICR-191	0.5		59 ± 1 [6.9]
WP2 uvrA	4NQO	0.5		167 ± 9 [7.7]

Test article: Extract of 70% extract of palm cactus stem
^aTwo plate/dose were used. No. of colonies of treated plate/No. of colonies of the negative control plate
Abbreviations: 2-AA: 2-aminoanthracene, SA: sodium azide, B[a]P: benzo[a]pyrene, ICR-191: acridine mutagen ICR 191, 4NQQ: 4-nitroqinoline N-oxide, 2-NF: 2-Nitrofluorene

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ISSN: 2662-7671

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