Fig. 3

Untreated cells (a) and HT-29 cells exposed to 100 μg/mL CPK-H (24 h) (b) and CAK-Tot extract (48 h) (c), 1000 μg/mL SPK-Tot (d) and 500 μg/mL SAK-H (72 h) extracts (e). HT-29 colon cancer cells, mostly found in interphase, form colonies of round to elliptical shapes. Round nuclei with 2–4 prominent nucleoli with uniform sized cells are visible. Treated HT-29 colon cancer cells (b) show membrane blebbing (arrows) indicative of apoptosis formation, hypercondensed chromatin (c) and cellular shrinkage (d and e). Figure 3a, c, d, and e (magnification 40x), and 3b (magnification 100x)