Table 4 HPLC-PDA method detection and quantification limit of eleven polyphenolic compounds with linearity
Compound name | Linearity range (μg/ml) | Detection limit (μg/ml) | Quantitative limit (μg/ml) | Regression equation, f(x) | Correlation coefficient (r) |
|---|---|---|---|---|---|
Gallic acid | 10.4–667 | 0.0109 | 0.0330 | 7.01 × 10−5(x) | 0.9998 |
Catechin | 10.4–667 | 0.0650 | 0.197 | 4.19 × 10−4(x) | 0.9999 |
Chlorogenic acid | 5.21–333 | 0.0202 | 0.0612 | 1.31 × 10−4(x) | 0.9999 |
Epicatechin | 10.4–667 | 0.0408 | 0.124 | 2.63 × 10−4(x) | 0.9999 |
Caffeic acid | 5.21–333 | 0.0111 | 0.0337 | 7.19 × 10−5(x) | 0.9999 |
Umbelliferone | 5.21–333 | 0.0330 | 0.100 | 2.14 × 10−4(x) | 0.9999 |
p-Coumaric acid | 5.21–333 | 0.00528 | 0.0160 | 3.41 × 10−5(x) | 0.9999 |
Rutin | 10.4–667 | 0.0398 | 0.121 | 2.58 × 10−4(x) | 0.9999 |
Ellagic acid | 5.21–333 | 0.0336 | 0.102 | 2.20 × 10−4(x) | 0.9999 |
Quercetin | 5.21–333 | 0.0213 | 0.0646 | 1.37 × 10−4(x) | 0.9999 |
Kaempferol | 5.21–333 | 0.0659 | 0.200 | 4.26 × 10−4(x) | 0.9996 |