Fig. 7
From: Suppression of lung inflammation by the ethanol extract of Chung-Sang and the possible role of Nrf2
Intratracheal eCS suppresses neutrophil infiltration in mouse lungs. Bronchoalveolar lavage (BAL) was performed with C57BL/6 mice (n = 5/group) treated as in Fig. 5. Total cells (a) and macrophages (open columns) and neutrophils (closed columns) (b) were counted in the BAL fluid. ***P was less than 0.0001, compared to the mice treated with LPS only. c After perfusion, lung lysate was prepared, with which MPO activity was measured. ***P was less than 0.0001, compared to the mice treated with LPS only. Data are presented as the mean ± SEM of 5 mice per group. d Total RNA was extracted from the lung, with which expressions of IL-6, IL-1β, and TNF-α were analyzed by a real-time quantitative PCR. *P and **P were less than 0.05 and 0.001, respectively, compared to the mice treated with LPS only