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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Danggui Buxue Tang, an ancient Chinese herbal decoction, protects β-amyloid-induced cell death in cultured cortical neurons

Fig. 5

DBT attenuates the Aβ-induced apoptosis markers. Cultured cortical neurons were co-treated with different amounts of DBT (DBT-L: 12.5 μg/mL; DBT-H: 100 μg/mL) and Aβ (20 μM) or directly treated with Aβ or DBT alone for 2 days. The translational levels of cleaved-PARP (cl-PARP at ~ 85 kDa), cleaved-caspase 3 (cl-caspase 3 at ~ 17 kDa) and cleaved-caspase 9 (cl-caspase 9 at ~ 37 kDa) were detected by immunoblot analysis by specific antibodies. Beta-actin (~ 42 kDa) served as an internal control. Quantification of target protein expression was calculated by a densitometer. Values were expressed as the percentage of change as compared to control reading, in Mean ± SEM, where n = 3. Statistically significant changes were classified as significant (*) where p < 0.05, more significant (**) where p < 0.01 and highly significant (***) where p < 0.001 as compared with control group. Significant (^) where p < 0.05 and more significant (^^) where p < 0.005 are compared with Aβ group

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