Fig. 8

JPYSF restores the level of mitophagy in 5/6 Nx rats. a Representative Western blot images show decreased PINK1 and p-Parkin expression in the 5/6 Nx group, which were restored by JPYSF treatment. b, c Densitometric analysis of PINK1 and p-Parkin protein expression normalized to GAPDH content. d Representative immunofluorescence images indicating the colocalization of Parkin and HSP-60. e Representative immunofluorescence images indicating the colocalization of LAMP-1 and HSP-60. Green corresponds to interest proteins, red corresponds to mitochondria, and blue corresponds to nuclear staining. All images are shown at identical magnification, × 200, scale bar = 100 μm. Data are presented as the means ± SEM, n = 6 rats per group. (*P < 0.05, **P < 0.01 compared with the sham group; ^#P < 0.05, ^##P < 0.01 compared with the 5/6 Nx group; ^δP < 0.05 compared with the 5/6 Nx + Perindopril group)