Fig. 4

JPYSF retards renal fibrosis in 5/6 Nx rats. a Representative Western blot images indicate that fibronectin, collagen IV, and α-SMA protein expression were significantly increased in the 5/6 Nx group but were abolished by JPYSF treatment. b-d Densitometric analysis of fibronectin, collagen IV, and α-SMA protein expression normalized to α-tubulin content. e Representative immunofluorescence images of fibronectin, collagen IV, and α-SMA. Green corresponds to interest proteins, and blue corresponds to nuclear staining. All images are shown at identical magnification, × 200, scale bar = 100 μm. Data are presented as the means ± SEM, n = 6 rats per group. (*P < 0.05, **P < 0.01 compared with the sham group; ^#P < 0.05, ^##P < 0.01 compared with the 5/6 Nx group)