Fig. 4

Effect of eugenol treatment on the mRNA expression of MMP2 in MDA-MB-231 and SK-BR-3 (a), and on MMP2 protein levels (b), mRNA expression of MMP9 in MDA-MB-231 and SK-BR-3 (c) and MMP 9 protein levels (d), mRNA expression of TIMP-1 in MDA-MB-231 and SK-BR-3 (e), and TIMP-1 protein levels (f). Cells were treated for 48 h with eugenol (4 and 8 μM and 5 and 10 μM for MDA-MB-231 and SK-BR-3, respectively). Data are presented as mean ± SD (n = 3). * and # indicate a significant change from untreated, 4, and 5 μM eugenol respectively, at p < 0.05 using ANOVA followed by Tukey–Kramer post-ANOVA test