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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Anti-metastatic and anti-proliferative activity of eugenol against triple negative and HER2 positive breast cancer cells

Fig. 2

a Proliferation curve of MDA-MB-231 cells obtained by real-time analysis. Cells were seeded in E-16 Plates at 2 × 104 cells/well and were continuously observed by measuring the cell index (CI) values for 72 h. Cell proliferation was observed at intervals of 15 min. The black line marks the normalization of the CI time point at 20 h. MDA-MB-231 cells were then treated with different doses of eugenol. Colored curves represent the various concentrations of eugenol. Pink line: Control (DMSO); Blue line: 4 μM Eugenol; Green line: 8 μM Eugenol; Red line: 10 μM Eugenol. Data are presented as mean ± SD (n = 3). *, # and $ indicate a significant change from untreated cells, 4, and 8 μM eugenol, respectively, at p < 0.05 using ANOVA followed by Tukey–Kramer post-ANOVA test. b Proliferation curve of SK-BR-3 cells obtained by real-time analysis. Cells were seeded in E-16 Plates at 2 × 104 cells/well and were continuously observed by measuring CI values for 72 h. Cell proliferation was observed at intervals of 15 min. The black line marks the normalization of the CI time point at 20 h. SK-BR-3 cells were then treated with different doses of eugenol. Colored curves represent various concentrations of eugenol. Pink line: Control (DMSO); Blue line: 5 μM Eugenol; Green line: 10 μM Eugenol; Red line: 12 μM Eugenol. Data are presented as mean ± SD (n = 3). * and # indicate significant changes from untreated and 5 μM eugenol, respectively, at p < 0.05 using ANOVA followed by Tukey–Kramer post-ANOVA test

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