Fig. 2

Effect of PHME on adipocyte lipolysis. a Fully differentiated adipocytes treated with various concentrations of PHME (100, 200, 300, 400 and 500 μg/mL) for 24, 48 and 72 h, respectively. Then, glycerol contents in media were measured by glycerol assay kit. b Adipocytes treated with PHME for 24, 48 and 72 h were stained with oil red O and quantified intracellular lipid by measuring the absorbance at 500 nm. Values are expressed as mean ± SD (n = 4), *P < 0.05 and **P < 0.01 vs. control group, respectively