Table 1 Mobile phase condition of chromatographic separation
Time (min) | Acetonitrile (%) | Methanol (%) | 0.5% acetic acid in water (%) |
|---|---|---|---|
0–30 | 12–25 | 0 | 88–75 |
30–45 | 25–23.5 | 0–11 | 75–65.5 |
45–68 | 23.5–35 | 11–4 | 65.5–61 |
68–85 | 35 | 4 | 61 |
85–90 | 35–50 | 4–0 | 61–50 |
90–95 | 100 | 0 | 0 |
95–110 | 100 | 0 | 0 |