Anti-osteoporotic effects of mixed compositions of extracellular polymers isolated from Aureobasidium pullulans and Textoria morbifera in ovariectomized mice

Cho, Chang-Soo; Jeong, Hye-Seong; Kim, In-Young; Jung, Go-Woon; Ku, Bon-Hwa; Park, Dong-Chan; Moon, Seung-Bae; Cho, Hyung-Rae; Bashir, Khawaja Muhammad Imran; Ku, Sae Kwang; Choi, Jae-Suk

doi:10.1186/s12906-018-2362-y

BMC Complementary Medicine and Therapies

Table 3 Bone femur strength, and mineral content (Ca and IP) in sham-operated and OVX mice

From: Anti-osteoporotic effects of mixed compositions of extracellular polymers isolated from Aureobasidium pullulans and Textoria morbifera in ovariectomized mice

Items Groups	Bone Mineral Density (g/cm²)		Strength (Newton)	Right femur bone mineral contents
Items Groups	Total body	Right femur	Right femur	Ca (mg/g bone)	IP (mg/g bone)	Ca/IP ratio
Controls
Sham	0.0249 ± 0.0007	0.0274 ± 0.0008	12.47 ± 1.66	59.08 ± 10.25	45.42 ± 7.57	1.30 ± 0.07
OVX	0.0215 ± 0.0003^f	0.0231 ± 0.0001^a	6.69 ± 0.91^f	33.37 ± 3.32^f	26.14 ± 3.26^f	1.28 ± 0.11
RES 2.5 mg/kg	0.0251 ± 0.0008^h	0.0262 ± 0.0013^bc	11.27 ± 1.78^h	55.33 ± 11.04^h	44.03 ± 10.17^h	1.26 ± 0.05
EAP 200 mg/kg	0.0233 ± 0.0003^fh	0.0252 ± 0.0007^ac	8.59 ± 0.63^fh	42.76 ± 4.14^fh	33.27 ± 3.18^fh	1.29 ± 0.06
TM 200 mg/kg	0.0231 ± 0.0004^fh	0.0248 ± 0.0006^ac	7.96 ± 0.69^fi	39.28 ± 3.84^fi	31.32 ± 3.51^fh	1.26 ± 0.06
Mixed formula - EAP:TM (200 mg/kg)
1:1	0.0333 ± 0.0003^fh	0.0253 ± 0.0006^ac	8.32 ± 0.98^fh	40.63 ± 2.92^fh	31.79 ± 2.93^fh	1.28 ± 0.07
1:3	0.0232 ± 0.0003^fh	0.0253 ± 0.0008^ac	8.04 ± 0.67^fi	40.77 ± 3.43^fi	32.39 ± 3.40^fi	1.26 ± 0.06
1:5	0.0231 ± 0.0003^fh	0.0250 ± 0.0010^ac	8.23 ± 0.86^fh	41.48 ± 4.10^fh	32.85 ± 3.64^fh	1.27 ± 0.09
1:7	0.0232 ± 0.0004^fh	0.0252 ± 0.0009^ac	8.08 ± 0.88^fh	40.39 ± 1.58^fh	31.51 ± 1.58^fh	1.28 ± 0.04
1:9	0.0232 ± 0.0003^fh	0.0249 ± 0.0008^ac	8.29 ± 0.84^fi	40.35 ± 4.00^fi	32.17 ± 3.02^fi	1.25 ± 0.05
3:1	0.0244 ± 0.0005^hjl	0.0268 ± 0.0011^cde	10.58 ± 1.17^ghjl	48.96 ± 2.87^hhjl	38.25 ± 2.96^ghkl	1.28 ± 0.06
5:1	0.0234 ± 0.0006^fh	0.0256 ± 0.0015^ac	8.48 ± 0.81^fh	42.67 ± 5.16^fh	33.66 ± 4.52^fh	1.27 ± 0.06
7:1	0.0232 ± 0.0004^fh	0.0249 ± 0.0010^ac	8.36 ± 0.54^fh	42.25 ± 3.11^fh	33.26 ± 3.28^fh	1.28 ± 0.09
9:1	0.0232 ± 0.0003^fh	0.0250 ± 0.0005^ac	8.45 ± 0.69^fh	40.21 ± 3.74^fh	31.61 ± 2.00^fh	1.27 ± 0.05

OVX Bilateral ovariectomy, RES Risedronate sodium, EAP Exopolymers purified from A. pullulans SM2001, TM T. morbifera leaf extracts, (EAP:TM) Mixed formula consisted of EAP and TM (g/g), Ca Calcium, IP Inorganic phosphorus
Values are expressed mean ± S.D. of eight mice
^ap < 0.01 and ^bp < 0.05 as compared with sham control by LSD test
^dp < 0.01 as compared with EAP single formula treated mice by LSD test
^cp < 0.01 as compared with OVX control by LSD test
^ep < 0.01 as compared with TM single formula treated mice by LSD test
^fp < 0.01 and ^gp < 0.05 as compared with sham control by MW test
^jp < 0.01 and ^kp < 0.05 as compared with EAP single formula treated mice by MW test
^hp < 0.01 and ⁱp < 0.05 as compared with OVX control by MW test
^lp < 0.01 as compared with TM single formula treated mice by MW test

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ISSN: 2662-7671

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