Study of the anti-allergic and anti-inflammatory activity of Brachychiton rupestris and Brachychiton discolor leaves (Malvaceae) using in vitro models

Table 3 Effect of the total extracts and fractions of B. rupestris and B. discolor on superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils

Sample	Superoxide anion generation^a	Elastase release^a
Sample	IC₅₀ (μg/mL)^b	IC₅₀ (μg/mL)^b
BRT	4.92 ± 1.47	3.82 ± 0.55
BRH	5.69 ± 0.80	3.73 ± 1.16
BRD	2.99 ± 0.73	1.98 ± 1.54
BRE	6.25 ± 3.10	2.71 ± 0.79
BRR	3.01 ± 1.91	> 10^c
BDT	4.73 ± 0.97	5.37 ± 1.23
BDH	6.25 ± 2.18	6.04 ± 2.32
BDD	0.78 ± 0.29	1.57 ± 0.84
BDE	5.22 ± 1.35	2.95 ± 1.08
genistein	0.41 ± 0.09	4.41 ± 1.99

^aIC₅₀ values, results are presented as mean ± SD (n = 3), compared with the control value (formyl-methionyl-leucyl-phenylalanine/cytochalasin B, fMLF/CB)
^bConcentration necessary for 50% inhibition (IC₅₀)
^cBRR exerted significant inhibitory activity in superoxide anion generation (49.6 ± 2.9%, **p < 0.001) at 10 μg/mL. BRT B. rupestris total methanol extract, BRH B. rupestris n-hexane fraction, BRD B. rupestris dichloromethane fraction, BRE B. rupestris ethyl acetate fraction, BRR B. rupestris remaining MeOH(aq) fraction, BDT B. discolor total methanol extract, BDH B. discolor n-hexane fraction, BDD B. discolor dichloromethane fraction, BDE B. discolor ethyl acetate fraction

ISSN: 2662-7671