Sample | % viability, RBL-2H3a | % inhibition of A23187-induced β-hexosaminidase releaseb |
---|
100 μg/mL | 10 μg/mL | 100 μg/mL |
---|
BRT | 99.0 ± 1.7 | 3.0 ± 5.2 | 25.7 ± 2.1** |
BRH | 96.7 ± 4.0 | 3.3 ± 5.8 | 39.0 ± 13.1** |
BRD | 95.3 ± 8.1 | 4.3 ± 7.5 | 19.0 ± 4.4* |
BRE | 97.7 ± 4.0 | 2.0 ± 3.5 | 7.0 ± 5.2 |
BRR | 99.0 ± 1.7 | 4.3 ± 5.1 | 3.7 ± 6.4 |
BDT | 99.0 ± 1.7 | 3.7 ± 6.4 | 16.0 ± 5.0* |
BDH | 99.7 ± 0.6 | 4.3 ± 7.5 | 30.3 ± 3.1** |
BDD | 100.0 ± 0.0 | 0.0 ± 0.0 | 44.0 ± 7.8** |
BDE | 100.0 ± 0.0 | 1.7 ± 2.9 | 0.3 ± 0.6 |
- aThe cytotoxicity of samples towards RBL-2H3 cells was evaluated using MTT viability assay and none of the samples showed any toxicity; results are presented as mean ± SD (n = 3)
- bDexamethasone (10 nM) was used as the positive control and inhibited 62.0 ± 9.5%** of A23187-induced β-hexosaminidase release in RBL-2H3 cells. Results are presented as mean ± SD (n = 3); *p < 0.05, **p < 0.001 compared with the control value (A23187 only)
- BRT B. rupestris total methanol extract, BRH B. rupestris n-hexane fraction, BRD B. rupestris dichloromethane fraction, BRE B. rupestris ethyl acetate fraction, BRR B. rupestris remaining MeOH(aq) fraction, BDT B. discolor total methanol extract, BDH B. discolor n-hexane fraction, BDD B. discolor dichloromethane fraction, BDE B. discolor ethyl acetate fraction