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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Acanthus ebracteatus leaf extract provides neuronal cell protection against oxidative stress injury induced by glutamate

Fig. 6

Effect of AE leaf extracts on expression of Nrf2-regulated antioxidant genes in glutamate-treated HT22 cells. (a) Quantitative real-time RT PCR analysis of EAAT3, Nqo1, and Gclm mRNA expression in untreated HT22 cells (control) or cells exposed to 5 mM glutamate alone or glutamate combined with either AEH or AEE at 50 μg/mL for 20 h. β-actin served as an internal control to normalize the mRNA expression levels. (b) Western blot analysis of EAAT3 protein in whole cell lysates isolated from untreated HT22 cells (control) or cells exposed to 5 mM glutamate alone or glutamate combined with 50 μg/mL AEE for 22 h. (c) Western blot analysis of Nrf2 protein in nuclear and cytoplasmic fractions isolated from HT22 cells exposed for 1 h to the similar treatment conditions as in (b). Lamin B1 and β-actin were used as endogenous loading controls to normalize the protein expression levels from nuclear and cytoplasmic fractions/whole cell lysates, respectively. Data are expressed as the means ± SD, #P < 0.05, ###P < 0.001 vs. control; *P < 0.05, **P < 0.01, ***P < 0.001 vs. glutamate alone

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