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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Acanthus ebracteatus leaf extract provides neuronal cell protection against oxidative stress injury induced by glutamate

Fig. 5

Effect of AE leaf extracts on glutamate-induced ROS accumulation in HT22 cells. (a) Representative confocal micrographs of DCF-derived fluorescence in untreated HT22 cells (control), or cells exposed to 5 mM glutamate alone or glutamate combined with either AEH or AEE at 50 μg/mL for 14 h. (b) Relative intracellular ROS levels of HT22 cells treated with 5 mM glutamate alone or with different concentrations of either AEH or AEE for 14 h, determined using a microplate reader. Data are expressed as the means ± SEM, ###P < 0.001 vs. control; ***P < 0.001 vs. glutamate alone

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