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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Acanthus ebracteatus leaf extract provides neuronal cell protection against oxidative stress injury induced by glutamate

Fig. 4

Effect of AE leaf extract on subcellular distribution of AIF in glutamate-treated HT22 cells. (a) Representative confocal photographs of immunofluorescence staining with an antibody specific for AIF (red) and nuclei counterstaining with DAPI (blue) of untreated HT22 cells (control; top panel) or cells exposed to 5 mM glutamate alone (middle panel) or glutamate combined with 50 μg/mL AEE (bottom panel) for 16 h. (b) Western blot analysis of AIF protein in nuclear and cytoplasmic fractions isolated from HT22 cells exposed to the similar treatment conditions as in (a). Lamin B1 and β-actin were used as endogenous loading controls to normalize the expression level of AIF protein from nuclear and cytoplasmic fractions, respectively. Data are expressed as the means ± SD, ##P < 0.01, ###P < 0.001 vs. control; *P < 0.05, ***P < 0.001 vs. glutamate alone

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