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Table 5 Groups of phytochemicals identified in MECN

From: The leaves of Crataeva nurvala Buch-Ham. modulate locomotor and anxiety behaviors possibly through GABAergic system

Phytochemicals

Names of the tests

Expected changes

Results

Alkaloids

Mayer’s test

Yellowish buff color precipitate

+

Hager’s test

Yellow crystalline precipitate

–

Wagner’s test

Brown or deep brown precipitate

+

Dragendorff’s test

Orange or orange-brown precipitate

+

Tannic acid test

Buff color precipitate

+

Tannins

Ferric chloride test

Blue green color

+

Alkaline reagent test

Yellow to red precipitate

+

Glycosides

General test

Yellow color

+

Test for glucoside

Production of brick-red precipitation

+

Carbohydrates

Molisch’s test

A red or reddish violet ring is formed at the junction of two layers, and on shaking a dark purple solution is formed

+

Barfoed’s test (general test for monosaccharides)

Red precipitate

+

Fehling’s test

A red or brick-red precipitate

+

Test for reducing sugar

A brick-red precipitate

+

Flavonoids

Hydrochloric acid reduction test

Red color

+