Fig. 4

I) B-TCE pretreatment normalized glutamate-induced increase in stress chaperones expression. a) Confocal micrographs of Control, Glutamate (2 mM), B-TCE (20 μg/mL) and B-TCE + Glu treated primary cerebellar neurons immunostained for HSP70 and Mortalin. b) Representative immunoblots and histograms showing relative expression of HSP70 and Mortalin. II) B-TCE pretreatment regulated the expression of cell cycle and pro-apoptotic proteins during glutamate exposure. a) Confocal micrographs of Control, Glutamate (2 mM), B-TCE (20 μg/mL) and B-TCE + Glu treated primary cerebellar neurons immunostained for Cyclin D1, PCNA and Bcl-xL. b) Representative immunoblots and histograms showing relative expression of Cyclin D1 and Bcl-xL where α-Tubulin was used as internal control. Data was compared between Control and other groups such as Glutamate, B-TCE and B-TCE + Glu (^*p ≤ 0.01) as well as Glutamate alone with B-TCE and B-TCE + Glu groups (^#p ≤ 0.01). Confocal Images were captured at 60X objective (Scale bar: 50 μm)