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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Identification of antitumoral agents against human pancreatic cancer cells from Asteraceae and Lamiaceae plant extracts

Fig. 5

Different phases of cell death after 24 h treatment with Asteraceae plants on MIA Paca-2. The % of apoptotic cells depending on Yarrow and Marigold SFE extracts (a) and the % of necrotic populations after those treatments (b). A representative flow cytometry diagram (c). Annexin V-FITC/PI double staining discriminates the live cells (Annexin V−/PI−; bottom left quadrant), early apoptotic cells (Annexin V+/PI−; bottom right quadrant), late apoptotic (Annexin V+/PI+; upper right quadrant), and necrotic or dead cells (Annexin V−/PI+; upper left quadrant). Data represent means ± S.E.M of at least four independent experiments each performed in triplicate. Asterisks indicate statistical differences in treated cells with respect to the control (non-treated cells, DMSO 0.1%). *p < 0.05; ***p < 0.001

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