Table 1 Tentative compounds detected in 80% ethanol extract of Phyllanthus amarus by LCMS analysis
Peak | Retention time (RT) | Molecular ion peak | MS2 fragment ions | Tentative compounds identified |
|---|---|---|---|---|
1 | 1.7 | 131 (M-H)− | 909, 688, 244, 134 | D-glutamine |
2 | 2.0 | 195 (M-H)− | 909, 678, 404, 204 | Gluconic acid |
3 | 2.1 | 228 (M-H)− | 897, 709, 405, 238, 129 | Naringenin |
4 | 2.1 | 191 (M-H)− | 935, 596, 381, 191 | Quinic acid |
5 | 2.6 | 133 (M-H)− | 913, 677, 412, 230, 134 | D-(+)-Malic acid |
6 | 2.9 | 321 (M-H)− | 711, 412, 248, 183 | Dinitramin |
7 | 3.1 | 128 (M-H)− | 122 | 4-oxoproline |
8 | 4.2 | 387 (M-H)− | 514, 387, 207 | galloyl glucopyroxidase |
9 | 6.5 | 169 (M-H)− | 169 | Gallic acid |
10 | 7.0 | 343 (M-H)− | 169 | trio-O-methylellagic acid |
11 | 7.9 | 633 (M-H)− | 633, 463, 301 | corilagin |
12 | 8.0 | 331 (M-H)− | 169 | tuberonic acid hexoside |
13 | 8.5 | 951 (M-H)− | 933, 765, 613, 463, 301 | geraniin |
14 | 8.9 | 965 (M-H)− | 933, 765, 609, 300 | Rutin |
15 | 9.2 | 197 (M-H)− | 125 | Syringic acid |
16 | 9.2 | 463 (M-H)− | 463, 300, 169 | Quercetine 3-D-glucoside |
17 | 9.5 | 206 (M-H)− | 873, 704, 558, 226, 147 | N-acetyl-D- phenylalanine |
18 | 9.7 | 247 (M-H)− | 247 | 7-Deshydroxypyrogallin4- carboxylic acid |
19 | 1.6 | 133 (M-H)+ | 663, 383, 132 | Maleamate |
20 | 1.8 | 222 (M-H)+ | 222 | Metaxalone |
21 | 1.9 | 184 (M-H)+ | 184 | 4-pyridoxic acid |
22 | 3.6 | 166 (M-H)+ | 120 | Stachydrine |
23 | 13.9 | 419 (M-H)+ | 419, 149 | Phyllanthin |